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KMID : 0380619780100010046
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Korean Journal of Food Science and Technology
1978 Volume.10 No. 1 p.46 ~ p.51
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Formation of Luteoskyrin by Penicillium islandicum
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Abstract
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One of yellowed rice toxins, luteoskyrin, was investigated with respect to its identification, quantitation and producibility by Penicillium islandicum isolated from deteriorated rice.
1) Luteoskyrin was best resolved by thin-layer chromatography with silica gel G plate impregnated with 0. 5 N oxalic acid and acetone : n-hexane : water (6 : 3 : 1.5, upper layer) solvent system.
The isolated yellow spot showed maximum absorption bands at 426 and 448 nm and changed to purple color upon exposure to sunlight for 2∼3 hours.
2) Detection limit for luteoskyrin was 4 ppm in elution-colorimetry and 0.1 ppm in densitometry after TLC. Assuming that the tolerance for luteoskyrin in rice is set below 3.68 ppm, densitometry is usable for its screening in grain samples
3) Producibility of luteoskyrin by Pan. islandicum was shown to be 11 ㎎/g mycelial mat in liquid culture and 40 ㎎/g autoclaved rice.
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